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CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
CellTiter-Glo? 細(xì)胞活力檢測(cè)試劑盒(CTG)
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經(jīng)典金標(biāo)準(zhǔn)細(xì)胞活力/增值/毒性檢測(cè)方法,是進(jìn)入了各大藥廠藥物研發(fā)SOP 多年的經(jīng)典系統(tǒng),擁有海量高分引文
僅需加入試劑的操作模式,最快10分鐘孵育檢測(cè)
高靈敏度,線性范圍寬,最低可檢測(cè)10個(gè)細(xì)胞
輕松兼容96-1536孔板及自動(dòng)化高通量篩選 (HTS)
| 產(chǎn)品名稱 | 規(guī)格 | 目錄號(hào) | 目錄價(jià) | 促銷價(jià) |
|---|---|---|---|---|
| CellTiter-Glo? Luminescent Cell Viability Assay | 10ml | G7570 | 837 | |
| CellTiter-Glo? Luminescent Cell Viability Assay | 10 × 10ml | G7571 | 4088 | |
| CellTiter-Glo? Luminescent Cell Viability Assay | 100ml | G7572 | 3465 | |
| CellTiter-Glo? Luminescent Cell Viability Assay | 10 × 100ml | G7573 | 26824 |
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技術(shù)原理
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CellTiter-Glo? Luminescent Cell Viability Assay (CellTiter-Glo?發(fā)光法細(xì)胞活力檢測(cè)試劑盒) 是通過對(duì)ATP 進(jìn)行定量測(cè)定來檢測(cè)培養(yǎng)物中活細(xì)胞數(shù)目的一種均質(zhì)快速檢測(cè)方法。其主要原理是利用螢光素酶在與甲蟲螢光素底物進(jìn)行發(fā)光反應(yīng)時(shí)需要ATP,而ATP的量與發(fā)光強(qiáng)度呈線性關(guān)系。
試劑盒中包括緩沖液及凍干底物,檢測(cè)前將緩沖液與檢測(cè)底物完全混合形成 CellTiter-Glo? 試劑,檢測(cè)步驟就是將單一試劑(CellTiter-Glo? 試劑) 直接加入含有血清的培養(yǎng)細(xì)胞中,無需洗滌細(xì)胞、去除培養(yǎng)基或進(jìn)行多步加樣操作。在384孔板上,加入試劑并混合后,10分鐘內(nèi),該系統(tǒng)可檢測(cè)到的每個(gè)孔內(nèi)的細(xì)胞數(shù)最低為15個(gè)。

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應(yīng)用數(shù)據(jù)
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CellTiter-Glo? Luminescent Cell Viability Assay 在很寬的細(xì)胞數(shù)范圍內(nèi)都呈現(xiàn)線性
左圖 . 細(xì)胞數(shù)與發(fā)光信號(hào)相關(guān)。 在 96 孔板中以 RPMI 1640 + 10% FBS 進(jìn)行 2 倍梯度稀釋 Jurkat 細(xì)胞。再加入 CellTiterGlo? Reagent,孵育 10 min 后檢測(cè)??梢娫?0-50000 個(gè)細(xì)胞/ 孔 (96 孔板 ) 的范圍內(nèi)與光信號(hào)呈線性相關(guān) (r2 = 0.99) 。
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ATP檢測(cè)及細(xì)胞活力快速檢測(cè)方案
細(xì)胞生物學(xué) | 技術(shù)介紹手冊(cè) |更新時(shí)間:2025/01/09語言: 中文
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PARP/DDR 通路藥物研發(fā)解決方案
小分子藥物研發(fā) | 產(chǎn)品簡(jiǎn)要介紹 |更新時(shí)間:2024/12/27語言: 中文
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脂類代謝檢測(cè)系統(tǒng)
細(xì)胞生物學(xué) | 產(chǎn)品簡(jiǎn)要介紹 |更新時(shí)間:2024/12/27語言: 中文
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Promega 細(xì)胞代謝檢測(cè)產(chǎn)品列表
細(xì)胞生物學(xué) | 產(chǎn)品或工具列表 |更新時(shí)間:2024/12/27語言: 中文
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細(xì)胞代謝檢測(cè)解決方案
細(xì)胞生物學(xué) | 技術(shù)介紹手冊(cè) |更新時(shí)間:2024/12/27語言: 中文
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AMP相關(guān)酶藥物靶標(biāo)高通量檢測(cè)技術(shù)
小分子藥物研發(fā) | 技術(shù)介紹手冊(cè) |更新時(shí)間:2024/12/27語言: 中文
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T細(xì)胞免疫治療研發(fā)解決方案
基因與細(xì)胞治療 | 技術(shù)介紹手冊(cè) |更新時(shí)間:2024/12/17語言: 中文
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抗體偶聯(lián)藥物(ADCs)研發(fā)用工具
大分子藥物研發(fā) | 技術(shù)介紹手冊(cè) |更新時(shí)間:2024/12/06語言: 中文
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細(xì)胞健康檢測(cè)解決方案
細(xì)胞生物學(xué) | 技術(shù)介紹手冊(cè) |更新時(shí)間:2024/12/04語言: 中文
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A Non-canonical PDK1-RSK Signal Diminishes Pro-caspase-8-Mediated Necroptosis Blockade
G7570,G7571,G7572,G7573,G8200,G8201,G8202,G8090
細(xì)胞活力/毒性/凋亡檢測(cè)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG) Caspase-Glo? 8 Assay Systems Caspase-Glo? 3/7 Assay System Caspase-Glo? 3/7 Assay Systems
關(guān)鍵詞:PDK1,TNF,caspase-8,程序性壞死,RSK磷酸化,zVAD
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Therapeutic targeting of the mevalonate–geranylgeranyl diphosphate pathway with statins overcomes chemotherapy resistance in small cell lung cancer
G8461,G8462,G7571,G7570,G7572,G7573
細(xì)胞自噬研究,細(xì)胞活力/毒性/凋亡檢測(cè),細(xì)胞信號(hào)通路研究,細(xì)胞代謝研究應(yīng)用產(chǎn)品: CellTiter-Glo? One Solution Assay CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
關(guān)鍵詞:細(xì)胞活力,小細(xì)胞肺癌,細(xì)胞信號(hào)通路,化療耐藥
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Preclinical characterization of the novel anti- sIRP α anti-ody BR105 that targets the mye1oid immune checkpoint
G7570,G7571,G7572,G7573
細(xì)胞活力/毒性/凋亡檢測(cè),藥物研發(fā)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
關(guān)鍵詞:免疫檢查點(diǎn),CD47-SIRPα,細(xì)胞活力檢測(cè)
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Inhibition of the FACT Complex Targets Aberrant Hedgehog Signaling and Overcomes Resistance to Smoothened Antagonists
G7570,G7571,G7572,G7573
細(xì)胞活力/毒性/凋亡檢測(cè),調(diào)控元件應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
關(guān)鍵詞:腫瘤治療靶點(diǎn),F(xiàn)ACT復(fù)合物,轉(zhuǎn)錄因子,細(xì)胞活力檢測(cè)
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Identification of the dietary supplement capsaicin as an inhibitor of Lassa virus entry
G7570,G7571,G7572,G7573,E1500,E1501,E4030,E4530,E4550
細(xì)胞活力/毒性/凋亡檢測(cè),病毒研究,藥物研發(fā)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG) Luciferase Assay System
關(guān)鍵詞:病毒進(jìn)入,辣椒素,細(xì)胞活力檢測(cè),螢光素酶報(bào)告基因檢測(cè)
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Echinatin effectively protects against NLRP3 inflammasome–driven diseases by targeting HSP90
G9951,G9952,G9953,G1780,G7570,G7571,G7572,G7573
細(xì)胞活力/毒性/凋亡檢測(cè),蛋白互作研究 ,免疫學(xué)檢測(cè),細(xì)胞信號(hào)通路研究應(yīng)用產(chǎn)品: Caspase-Glo? 1 Inflammasome Assay CytoTox 96?Non-Radioactive Cytotoxicity Assay CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
關(guān)鍵詞:熱休克蛋白,NLRP3炎性小體,細(xì)胞活力檢測(cè),細(xì)胞毒性檢測(cè)
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Hexokinase 3 enhances myeloid cell survival via non-glycolytic functions
N3030,N3040,N3050,N2410,G8090,G8091,G8092,G8093,G7570,G7571,G7572,G7573
骨髓細(xì)胞分化,白血病應(yīng)用產(chǎn)品: Nano-Glo? HiBiT Lytic Detection System Nano-Glo? HiBiT Blotting System Caspase-Glo? 3/7 Assay System Caspase-Glo? 3/7 Assay Systems CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
關(guān)鍵詞:CRISPR/Cas9, HiBiT蛋白標(biāo)簽,糖酵解,骨髓細(xì)胞
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Influenza A Virus Polymerase Recruits the RNA Helicase DDX19 to Promote the Nuclear Export of Viral mRNAs
G7570,G7571,G7572,G7573,E2810,E2820
病毒研究應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG) Renilla Luciferase Assay System
關(guān)鍵詞:甲型流感病毒(IAV),抗病毒藥物,病毒-宿主相互作用,DExD-box RNA解旋酶(DDX)
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ALDOC- and ENO2- driven glucose metabolism sustains 3D tumor spheroids growth regardless of nutrient environmental conditions: A multi-omics analysis
G7570,G7571,G7572,G7573
細(xì)胞健康,細(xì)胞活力與細(xì)胞毒性檢測(cè)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
關(guān)鍵詞:metastasis, lung cancer, breast cancer, glucose metabolism, ALDOC, ENO2, tumor spheroids, omics
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High-pressure oxygen rewires glucose metabolism of patient-derived glioblastoma cells and fuels inflammasome response
G7570,G7571,G7572,G7573
細(xì)胞健康,細(xì)胞活力與細(xì)胞毒性檢測(cè)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
關(guān)鍵詞: Glioblastoma, hypoxia, inflammation, radioresistance, preclinical studies
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Elevated expression of miR-494-3p is associated with resistance to osimertinib in EGFR T790M-positive non-small cell lung cancer
G7570,G7571,G7572,G7573,G8090,G8091,G8092,G8093
細(xì)胞活力與細(xì)胞毒性檢測(cè),細(xì)胞活力/毒性/凋亡檢測(cè)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG) Caspase-Glo? 3/7 Assay System Caspase-Glo? 3/7 Assay Systems
關(guān)鍵詞:non-small cell lung cancer (NSCLC), epidermal growth factor receptor (EGFR), osimertinib, microRNA (miRNA); tyrosine kinase inhibitor (TKI)
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Metformin attenuates rotenone-induced oxidative stress and mitochondrial damage via the AKT/Nrf2 pathway
G3580,G3581,G3582,G8090,G8091,G8092,G8093,G7570,G7571,G7572,G7573,V6911,V6912
細(xì)胞健康,細(xì)胞活力與細(xì)胞毒性檢測(cè)應(yīng)用產(chǎn)品: CellTiter 96? AQueous One Solution Cell Proliferation Assay (MTS) Caspase-Glo? 3/7 Assay System Caspase-Glo? 3/7 Assay Systems CellTiter-Glo? Luminescent Cell Viability Assay(CTG) GSH-Glo?Glutathione Assay GSH-Glo? Glutathione Assay
關(guān)鍵詞:metformin, rotenone, mitochondria, oxidative stress, Parkinson's disease
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Insights into the modular design of kinase inhibitors and application to Abl and Axl
G7570,G7571,G7572,G7573
細(xì)胞健康,細(xì)胞活力與細(xì)胞毒性檢測(cè)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG)
關(guān)鍵詞:kinase inhibitors, kinase target engagement, Abl, Axl
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Overcoming preclinical safety obstacles to discover GDC-2394: A potent and selective NLRP3 inhibitor
G7570,G7571,G7572,G7573,G9951,G9952,G9953
細(xì)胞健康,細(xì)胞活力與細(xì)胞毒性檢測(cè)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG) Caspase-Glo? 1 Inflammasome Assay
關(guān)鍵詞:inflammasome, NLRP3, caspase-1, MCC950, NLRP3 inhibitor
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Comparative characterization of different molecular formats of bispecific antibodies targeting EGFR and PD-L1
G7570,G7571,G7572,G7573,J1252,GA1130
細(xì)胞健康,細(xì)胞活力與細(xì)胞毒性檢測(cè)應(yīng)用產(chǎn)品: CellTiter-Glo? Luminescent Cell Viability Assay(CTG) PD-1/PD-L1 Blockade Bioassays Control Ab, Anti-CD20
關(guān)鍵詞:bispecific antibodies, EFGR, PD-L1, triple-negative breast cancer (TNBC), bioassays
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電話: 400-810-8133
儲(chǔ)存條件:若需長(zhǎng)期儲(chǔ)存,則應(yīng)將 CellTiter-Glo? 底物凍干粉和 CellTiter-Glo?Buffer 置于 –20℃條件下儲(chǔ)存。若需頻繁使用,可將CellTiter-Glo?Buffer 置于 4℃或室溫下儲(chǔ)存 48 小時(shí)(活性不會(huì)降低)。關(guān)于有效期等信息,請(qǐng)參見產(chǎn)品標(biāo)簽。
復(fù)溶后 CellTiter-Glo?Reagent(緩沖液加底物)最長(zhǎng)可于室溫儲(chǔ)存 8 小時(shí)(活性降低程度 <10%),若于 4℃儲(chǔ)存 48 小時(shí),則活性降低程度約為5%,若于4℃儲(chǔ)存4天,則活性降低程度約20%,若于


